Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Complex Protein (Monomer, MALS verified)

Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms and binds copper, nickel, fatty acids, and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. The Human HLA-A*0201 AFP (FMNKFIYEI) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and FMNKFIYEI peptide of the AFP.

Product Specifications

Background

Specifications

Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Complex Protein is produced by co-expression of HLA and B2M loaded with AFP peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 36.3 kDa, 11.7 kDa and 1.2 kDa. The protein migrates as 41-45 kDa and 12 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAA59606.1 (HLA-A*02:01) & P61769 (B2M) & FMNKFIYEI

Expression Region

Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & FMNKFIYEI peptide

Host

HEK293

Target

HLA-A*0201 & B2M & AFP (FMNKFIYEI)

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Complex Protein (Cat. No. HLA-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).