Biotinylated Human HLA-A*02:01&B2M&EW (QLQNPSYDK) Complex Protein (Monomer, MALS verified)

Biotinylated Human HLA-A*02:01&B2M&EW (QLQNPSYDK) Complex Protein (HLE-H82E4) is expressed from human 293 cells (HEK293) . It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & QLQNPSYDK peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & QLQNPSYDK) .

Product Specifications

Specifications

Biotinylated Human HLA-A*02:01&B2M&EW (QLQNPSYDK) Complex Protein is produced by co-expression of HLA and B2M loaded with EW peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 36.3 kDa and 11.7 kDa. The protein migrates as 40-44 kDa and 13 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & QLQNPSYDK

Expression Region

Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & QLQNPSYDK peptide

Host

HEK293

Target

HLA-A*0201 & B2M & EW (QLQNPSYDK)

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Biotinylated Human HLA-A*02:01&B2M&EW (QLQNPSYDK) Complex Protein (Cat. No. HLE-H82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-4 ng/mL (QC tested).