Human Integrin alpha 6 beta 4 (ITGA6&ITGB4) Heterodimer Protein, His Tag&Tag Free (MALS verified)

Human integrin alpha (10) I domain as a recombinant protein to reveal its ligand binding specificity. In general, alpha (10) I did recognize collagen types I-VI and laminin-1 in a Mg (2+) -dependent manner, whereas its binding to tenascin was only slightly better than to albumin. Alpha 10 beta 1 is a known collagen-binding I domain integrin, in addition to α1β1, α2β1 and α11β1. GROGER found in the N-terminal domain of collagens I and III, is only weakly recognised by α10β1, an important collagen receptor on chondrocytes, contrasting with the other collagen-binding integrins.

Product Specifications

Background

Specifications

Human ITGA6&ITGB4 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA6 and ITGB4, has a calculated MW of 116.8 kDa (ITGA6) and 81.8 kDa (ITGB4) . Subunit ITGA6 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB4 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 130-140 kDa (ITGA6) and 75-85 kDa (ITGB4) when calibrated against Star Ribbon Pre-stained Protein Marker respectively due to glycosylation.

Accession Number

P23229-2 & P16144-2

Expression Region

Phe 24 - Gly 1012 | Asn 28 - Ser 710

Host

HEK293

Target

Integrin alpha 6 beta 4

Conjugation

Unconjugated

Tag

C-10xHis | Native

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human Integrin alpha 6 beta 4 Protein, His Tag&Tag Free (Cat. No. IN4-H52W3) at 5 μg/mL (100 μL/well) can bind Human Laminin 511 Protein, premium grade (Cat. No. LA8-H5283) with a linear range of 0.8-13 ng/mL (QC tested).