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Cynomolgus ALPP Protein, His Tag (active enzyme)

Cynomolgus ALPP, His Tag (ALP-C52H3) is expressed from human 293 cells (HEK293) . It contains AA Ile 21 - Asp 504 (Accession # XP_045223825.1) .

Product Specifications

Background

In most mammals there are four different isozymes: placental (ALPP), germ cell (ALPG), intestinal (ALPI) and tissue non-specific (liver/bone/kidney) (ALPL/TNAP) . ALPP is also known as Alkaline phosphatase, placental type and PLAP. Alkaline phosphatase that can hydrolyze various phosphate compounds.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 54.6 kDa. The protein migrates as 65-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

XP_045223825.1

Expression Region

Ile 21 - Asp 504

Host

HEK293

Target

ALPP

Conjugation

Unconjugated

Tag

C-10xHis

Source

Cynomolgus

Stability

● The product MUST be stored at -70°C or lower upon receipt; ● -70°C for 3 months under sterile conditions.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Cynomolgus ALPP, His Tag (Cat. No. ALP-C52H3) at 1 μg/mL (100 μL/well) can bind various dilution ratio of Anti-ALPP/ALPG/ALPI Polyclonal Antibody (QC tested).

Format

Liquid

Buffer

50 mM Tris, 150 mM NaCl, pH7.4

Additives

Trehalose

Molecular Weight

54.6 kDa

Shipping Conditions

Dry ice

Storage Conditions

-70°C

Package Size

100ug*1

Product Datasheet

Https://www.acrobiosystems.com/static/main/products/pdf/ds/ALP-C52H3.pdf

Species

Cynomolgus

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