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FITC-Labeled Human respiratory syncytial virus A (strain A2) Pre-F0 Protein, His Tag

Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. The RSV fusion glycoprotein (RSV F) is the principal target of RSV neutralizing antibodies in human sera. The RSV F is a type I viral fusion protein synthesized as inactive, single-chain polypeptides that assemble into trimers. RSV F fuses the viral and host cell membranes by irreversible protein refolding from the labile prefusion conformation to the stable post-fusion conformation.

Product Specifications

Background

Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. The RSV fusion glycoprotein (RSV F) is the principal target of RSV neutralizing antibodies in human sera. The RSV F is a type I viral fusion protein synthesized as inactive, single-chain polypeptides that assemble into trimers. RSV F fuses the viral and host cell membranes by irreversible protein refolding from the labile prefusion conformation to the stable post-fusion conformation.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 56.1 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P03420

Expression Region

Gln 26- Leu 513

Host

HEK293

Target

Prefusion glycoprotein F0/pre-F protein (RSV)

Conjugation

FITC Labeled

Tag

C-10xHis

Source

Human respiratory syncytial virus A (strain A2)

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Anti-Fusion glycoprotein F0 Antibody, Human IgG1 (D25) at 2 μg/mL (100 μL/well) can bind FITC-Labeled Human respiratory syncytial virus A (strain A2) Pre-F0, His Tag (Cat. No. RSF-V52H3) with a linear range of 0.02-0.625 μg/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

56.1 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C, avoid light

Package Size

250ug*2

Host or Source

HEK293

Species

Human respiratory syncytial virus A (strain A2)

Protein ID

P03420

Preservative

Trehalose

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