Cynomolgus LGR5 / GPR49 protein, His Tag
LGR5 (also known as GPR49) is a seven-transmembrane protein of the class A Rhodopsin-like family of GPCRs. LGR5 and LGR4 bind the R-spondins with high affinity and mediate the potentiation of Wnt/β-catenin signaling by enhancing Wnt-induced LRP6 phosphorylation. The LGR5/RSPO complex could promote Wnt signalling via the neutralization of two transmembrane E3 ligases, RNF43 and ZNRF3.
Product Specifications
Background
LGR5 (also known as GPR49) is a seven-transmembrane protein of the class A Rhodopsin-like family of GPCRs. LGR5 and LGR4 bind the R-spondins with high affinity and mediate the potentiation of Wnt/β-catenin signaling by enhancing Wnt-induced LRP6 phosphorylation. The LGR5/RSPO complex could promote Wnt signalling via the neutralization of two transmembrane E3 ligases, RNF43 and ZNRF3.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 61.7 kDa. The protein migrates as 66-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
G7PI19-1
Expression Region
Ser 22 - Gly 557
Host
HEK293
Target
LGR5
Conjugation
Unconjugated
Tag
C-10xHis
Source
Cynomolgus
Stability
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
95%
Bioactivity
Immobilized Cynomolgus LGR5, His Tag (Cat. No. LG5-C52H7) at 5 μg/mL (100 μL/well) can bind Human R-Spondin 1 (21-146), Fc Tag (Cat. No. RS1-H5269) with a linear range of 0.2-5 ng/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
61.7 kDa
Additionnal Information
Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
500ug*2
Host or Source
HEK293
Species
Cynomolgus
Protein ID
G7PI19-1
Preservative
Trehalose
More Discoveries
Explore Other Products
Browse additional items from our catalog
P3105-23C-ML750 100 µL
Papilloma Virus, Human, Type 18, E6 (hPV, Human Papillomavirus Type 18 E6, E6, Early Protein E6, HpV18gp1, Protein E6) (MaxLight 750) discontinued
Sign In for Pricing
View Details 
TRC-Z701495-5MG 5 mg
Zuclopenthixol (-)-10-Camphorsulfonic Acid Salt
Sign In for Pricing
View Details 
C9697 96 Tests
DnaJ homolog subfamily A member 4 (DNAJA4) Human ELISA Kit
Sign In for Pricing
View Details 