Biotinylated Human HLA-A*02:01&B2M Monomer Protein (Peptide free, MALS verified)
Biotinylated Human HLA-A*02:01&B2M Monomer Protein (HLM-H82W3) is expressed from human 293 cells (HEK293) . It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) (Accession # AAA59606.1 (HLA-A*02:01) & P61769 (B2M) ) .
Product Specifications
Specifications
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 36.3 kDa and 11.7 kDa. The protein migrates as 40-43 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
AAA59606.1 (HLA-A*02:01) & P61769 (B2M)
Expression Region
Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M)
Host
HEK293
Target
HLA-A*02:01 & B2M
Conjugation
Biotin-labeled
Tag
C-10xHis & C-Avi
Source
Human
Stability
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
0.1 EU per μg
Purity
95%
Bioactivity
Immobilized Biotinylated Human HLA-A*02:01&B2M Monomer Protein (Cat. No. HLM-H82W3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.00003-0.03 ng/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
36.3 kDa and 11.7 kDa
Additionnal Information
Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
200ug*1
Host or Source
HEK293
Species
Human
Protein ID
AAA59606.1 (HLA-A*02:01) & P61769 (B2M)
Preservative
Trehalose
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