Biotinylated Human ALPG / ALPPL2 Protein, His, Avitag™ (MALS verified)

ALPPL2 is specifically highly expressed in naïve pluripotent stem cells. Moreover, in naïve state somatic cell reprogramming, naïve-primed transformation and other systems, the use of the ALPPL2 promoter-driven fluorescent reporter system can specifically indicate the establishment or withdrawal of naïve state pluripotency, thereby identifying ALPPL2 as naïve state pluripotent. specific cell surface markers. Some studies have found that knockout of ALPPL2 can specifically affect the establishment, stability and maintenance of naïve state pluripotency, proving that ALPPL2 protein is a functional naïve state surface molecular marker.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 56.2 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P10696-1

Expression Region

Ile 20 - Asp 503

Host

HEK293

Target

ALPG

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Biotinylated Human ALPG Protein, His,Avitag (Cat. No. ALG-H52E1) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti - ALPG / ALPPL2 with a linear range of 0.1-4 ng/mL (QC tested).