Rat Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein, His Tag&Tag Free (MALS verified)

Integrin alpha V beta 6 is a heterodimer of beta-6 associating with alpha-V. Integrin alpha-V beta-6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. Also, Integrin alpha-V beta-6 acts as a receptor for coxsackievirus A9 and coxsackievirus B1 as well as herpes simplex virus-1/HHV-1. Furthermore, it binds the TGF-beta latency‑associated peptide (LAP) and activates TGF-beta 1 or TGF-beta 3 from large latent complexes. This activation requires interaction with LTBP-1 and fibronectin, and is enhanced by PAR-1.

Product Specifications

Background

Specifications

Rat ITGAV&ITGB6 Heterodimer, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB6, has a calculated MW of 112.3 kDa (ITGAV) & 80.1 kDa (ITGB6) . Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB6 contains no tag but a basic tail at the C-terminus. The protein migrates as 125-145 kDa (ITGAV) and 70-85 kDa (ITGB6) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Accession Number

NP_001385621.1 (ITGAM) & Q6AYF4 (ITGB6)

Expression Region

Phe 31 - Pro 988 (ITGAV) & Gly 22 - Pro 708 (ITGB6)

Host

HEK293

Target

Integrin alpha V beta 6

Conjugation

Unconjugated

Tag

C-10xHis | Native

Source

Rat

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human Fibronectin at 5 μg/mL (100 μL/well) can bind Rat ITGAV&ITGB6 Heterodimer, His Tag&Tag Free (Cat. No. IT6-R52E6) with a linear range of 0.02-0.625 μg/mL (QC tested).