Biotinylated Mouse Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein, His, Avitag™&Tag Free (MALS verified)

Integrin alpha V beta 8 (ITGAV & ITGB8 or ITGAVB8) is expressed in yolk sac, placenta, brain perivascular astrocytes, Schwann cells, renal glomerular mesangial cells and pulmonary epithelial cells. Unlike other alpha V integrins, ITGAVB8 does not appear to assume different activation states, and the cytoplasmic tail does not connect to the cytoskeleton. It binds ligands containing an RGD motif, including vitronectin, fibrin and the latency associated peptide (LAP) of the latent TGF-beta complex. High affinity binding of alpha V beta 8 to LAP allows proteolytic cleavage by MT1-MMP, which releases active TGF-beta. This mechanism differs from that of alpha V beta 6, the other alpha V integrin which can activate TGF-beta from latency through non-proteolytic mechanisms. Downstream effects of TGF-beta activation include control of cell growth and associated vascularization.

Product Specifications

Background

Specifications

Biotinylated Mouse ITGAV&ITGB8 Heterodimer Protein is produced by co-expression of ITGAV and ITGB8, has a calculated MW of 114.2 kDa (ITGAV) & 77.5 kDa (ITGB8) . Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB8 contains no tag but a basic tail at the C-terminus.The protein migrates as 135-145 kDa (ITGAV) and 75-85 kDa (ITGB8) under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Accession Number

P43406-1 & Q0VBD0-1

Expression Region

Phe 31 - Val 988 & Gly 22 - Leu 681

Host

HEK293

Target

Integrin alpha V beta 8

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi | Native

Source

Mouse

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Mouse Latent TGFB1, His Tag (Cat. No. TG1-M5245) at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse ITGAV&ITGB8 Heterodimer Protein (Cat. No. IT8-M52W7) with a linear range of 1-20 ng/mL (QC tested).