Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Rat Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein, His Tag&Tag Free

Integrin alpha V beta 5 (ITGAV & ITGB5) is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, embryonic stem cells, and select endothelium and epithelium. ITGAV & ITGB5 binds ligands containing an RGD motif, notably vitronectin. Growth factors that increase PKC activity, such as VEGF or TGF alpha, promote ITGAV & ITGB5-mediated angiogenesis while alpha V beta 3, which may be expressed in the same cell, responds to FGF-basic and TNF alpha. An inhibitor of both down regulates tumor angiogenesis. During lung inflammation, up regulation of ITGAV & ITGB5 on myofibroblasts or infiltrating lymphocytes may contribute to fibrosis by freeing TGF beta from latency.

Product Specifications

Background

Integrin alpha V beta 5 (ITGAV & ITGB5) is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, embryonic stem cells, and select endothelium and epithelium. ITGAV & ITGB5 binds ligands containing an RGD motif, notably vitronectin. Growth factors that increase PKC activity, such as VEGF or TGF alpha, promote ITGAV & ITGB5-mediated angiogenesis while alpha V beta 3, which may be expressed in the same cell, responds to FGF-basic and TNF alpha. An inhibitor of both down regulates tumor angiogenesis. During lung inflammation, up regulation of ITGAV & ITGB5 on myofibroblasts or infiltrating lymphocytes may contribute to fibrosis by freeing TGF beta from latency.

Specifications

Rat ITGAV&ITGB5 Heterodimer, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB5, has a calculated MW of 112.3 kDa (ITGAV) & 81.6 kDa (ITGB3) . Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB3 contains no tag but a basic tail at the C-terminus. The protein migrates as 125-145 kDa (ITGAV) and 85-95 kDa (ITGB3) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Accession Number

NP_001385621.1 (ITGAM) & NP_671480.2 (ITGB5)

Expression Region

Phe 31 - Pro 988 (ITGAM) & Leu 25 - Asn 719 (ITGB5)

Host

HEK293

Target

Integrin alpha V beta 5

Conjugation

Unconjugated

Tag

C-10xHis | Native

Source

Rat

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human Vitronectin at 5 μg/mL (100 μL/well) can bind Rat ITGAV&ITGB5 Heterodimer, His Tag&Tag Free (Cat. No. IT5-R52E3) with a linear range of 0.02-0.156 μg/mL (QC tested).

Format

Powder

Buffer

50 mM Tris, 150 mM NaCl, pH7.5

Additives

Trehalose

Molecular Weight

112.3 kDa | 81.7 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

500ug*2

Host or Source

HEK293

Species

Rat

Protein ID

NP_001385621.1 (ITGAM) & NP_671480.2 (ITGB5)

Preservative

Trehalose

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

Mouse Monoclonal CD99 Antibody (SPM596) [DyLight 405]
NBP3-11459V 0.1 mL

Mouse Monoclonal CD99 Antibody (SPM596) [DyLight 405]

Ask
View Details
HDAC5 (HD5, FLJ90614, KIAA0600, NY-CO-9, Histone Deacetylase 5) (PE) discontinued
H1827-65-PE 100 µL

HDAC5 (HD5, FLJ90614, KIAA0600, NY-CO-9, Histone Deacetylase 5) (PE) discontinued

Ask
View Details
SNORD111 AAV Vector (Human) (CMV)
44688101 1 µg

SNORD111 AAV Vector (Human) (CMV)

Ask
View Details
5-bromo-2- (prop-2-ynyloxy) benzaldehyde
sc-278085 1 g

5-bromo-2- (prop-2-ynyloxy) benzaldehyde

Ask
View Details
FR 901379
276863-01 10 mg

FR 901379

Ask
View Details
FR 901379
276863-02 25 mg

FR 901379

Ask
View Details