HRP-Human CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Tag Free
HRP-Human CD3E & CD3D Heterodimer Protein, His Tag&Tag Free (CDD-HR2W3) is expressed from human 293 cells (HEK293) . It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1 (CD3E) & P04234-1 (CD3D) ) .
Product Specifications
Background
T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Specifications
HRP-Human CD3E & CD3D Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3D, has a calculated MW of 16.6 kDa (CD3E) and 13.4 kDa (CD3D) . Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D contains no tag.
Accession Number
P07766-1 (CD3E) & P04234-1 (CD3D)
Expression Region
Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D)
Host
HEK293
Target
CD3E & CD3D
Conjugation
HRP Labeled
Tag
C-8xHis | Native
Source
Human
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Bioactivity
Immobilized Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a) at 1 μg/mL (100 μL/well) can bind HRP-Human CD3E & CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-HR2W3) with a linear range of 0.1-8 ng/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
16.6 kDa (CD3E) & 13.4 kDa (CD3D)
Shipping Conditions
RT
Storage Conditions
-20°C, aviod light
Package Size
200ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/CDD-HR2W3.pdf
Species
Human
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