Bat SARS-like coronavirus Khosta-2 Spike Trimer Protein (R666A, KV966-967PP), His Tag
Bat SARS-like coronavirus Khosta-2 Spike Trimer, His Tag (SPN-B52H6) is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. It contains AA Gln 16 - Pro 1193 (Accession # A0A8E6HRK4 (R666A, KV966-967PP) ) .
Product Specifications
Background
The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 136.0 kDa. The protein migrates as 170-200 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
A0A8E6HRK4 (R666A, KV966-967PP)
Expression Region
Gln 16 - Pro 1193
Host
HEK293
Target
Spike protein
Conjugation
Unconjugated
Tag
C-10xHis
Source
Bat coronavirus
Stability
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
95%
Bioactivity
Immobilized Bat SARS-like coronavirus Khosta-2 Spike Trimer, His Tag (Cat. No. SPN-B52H6) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.039-0.625 μg/mL (QC tested).
Format
Powder
Buffer
0.1 M Sodium citrate, pH5.5
Additives
Trehalose
Molecular Weight
136.0 kDa
Additionnal Information
Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
50ug*1
Host or Source
HEK293
Species
Bat coronavirus
Protein ID
A0A8E6HRK4 (R666A, KV966-967PP)
Preservative
Trehalose
Curated Selection
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