Rhinolophus affinis coronavirus isolate LYRa11 Spike Trimer Protein (R671A, KV972-973PP), His Tag (MALS verified)

Rhinolophus affinis coronavirus isolate LYRa11 Spike Trimer, His Tag (SPN-R52H3) is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. It contains AA Asp 18 - Pro 1199 (Accession # A0A023PUW9 (R671A, KV972-973PP) ) .

Product Specifications

Background

The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 136.2 kDa. The protein migrates as 160-190 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

A0A023PUW9 (R671A, KV972-973PP)

Expression Region

Asp 18 - Pro 1199

Host

HEK293

Target

Spike protein

Conjugation

Unconjugated

Tag

C-10xHis

Source

Rhinolophus affinis coronavirus isolate LYRa11

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind Rhinolophus affinis coronavirus isolate LYRa11 Spike Trimer, His Tag (Cat. No. SPN-R52H3) with a linear range of 1-39 ng/mL (QC tested).