Human IL-23 alpha & IL-12 beta Heterodimer Protein, premium grade

Interleukin-23 subunit alpha (IL-23 alpha) can associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

Product Specifications

Background

Specifications

This protein carries no "tag". The protein has a calculated MW of 55.0 kDa. The protein migrates as 58 kDa±3 kDa under reducing (R) condition, and 58 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Accession Number

P29460-1 (IL-12B) & Q9NPF7-1 (IL-23A)

Expression Region

Ile 23 - Ser 328 (IL-12B) & Arg 20 - Pro 189 (IL-23A)

Host

HEK293

Target

IL23A & IL12B

Conjugation

Unconjugated

Tag

Native

Source

Human

Stability

-20°C to -70°C for 24 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.1 EU per μg

Purity

95%

Bioactivity

Immobilized Human IL-23A&IL-12B Heterodimer Protein, premium grade (Cat. No. ILB-H5219) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human IL23A&IL12B P40 domain Antibody, Human IgG1 with a linear range of 0.02-1 ng/mL (QC tested).