Human GDNF / ATF / hGDNF Protein, premium grade

Glial cell line-derived neurotrophic factor (GDNF) is a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. GDNF is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. The GDNF family members (consists of GDNF, neurturin, artemin and persephin) are structurally similar to transforming growth factor-beta. Unlike other members of the TGF-beta superfamily, which signal through the receptor serine-threonine kinases, GDNF family ligands activate intracellular signalling cascades via the receptor tyrosine kinase Ret. GDNF family ligands binds to GDNF family receptor alpha (GFRalpha) and then the GDNF family ligand-GFR alpha complex binds to and stimulates autophosphorylation of Ret.

Product Specifications

Background

Specifications

This protein carries no "tag". The protein has a calculated MW of 15.1 kDa. The protein migrates as 15-22 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P39905-1

Expression Region

Ser 78 - Ile 211

Host

HEK293

Target

GDNF

Conjugation

Unconjugated

Tag

Native

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.1 EU per μg

Purity

90%

Bioactivity

Immobilized Human GDNF Protein, premium grade (Cat. No. GDF-H5219) at 2 μg/mL (100 μL/well) can bind Human GFR alpha-1, His Tag (Cat. No. GF1-H5224) with a linear range of 0.2-4 ng/mL (QC tested).