Biotinylated Mouse Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein, His, Avitag™&Tag Free

Integrin alpha V beta 5 (ITGAV & ITGB5) is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, embryonic stem cells, and select endothelium and epithelium. ITGAV & ITGB5 binds ligands containing an RGD motif, notably vitronectin. Growth factors that increase PKC activity, such as VEGF or TGF alpha, promote ITGAV & ITGB5-mediated angiogenesis while alpha V beta 3, which may be expressed in the same cell, responds to FGF-basic and TNF alpha. An inhibitor of both down regulates tumor angiogenesis. During lung inflammation, up regulation of ITGAV & ITGB5 on myofibroblasts or infiltrating lymphocytes may contribute to fibrosis by freeing TGF beta from latency.

Product Specifications

Background

Specifications

Biotinylated Mouse ITGAV&ITGB5 Heterodimer Protein is produced by co-expression of ITGAV and ITGB5, has a calculated MW of 114.2 kDa (ITGAV) & 77.5 kDa (ITGB5) . Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB5 contains no tag but a basic tail at the C-terminus.The protein migrates as 80-90 kDa (ITGB5) and 125-145 kDa (ITGAV) under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Accession Number

P43406-1 & O70309-1

Expression Region

Phe 31 - Val 988 & Gly 24 - Asn 719

Host

HEK293

Target

Integrin alpha V beta 5

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi | Native

Source

Mouse

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human Fibronectin at 50 μg/mL (100 μL/well) can bind Biotinylated Mouse ITGAV&ITGB5 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT5-M82W9) with a linear range of 0.625-10 μg/mL (Routinely tested).