Biotinylated Human LTBR / TNFRSF3 Protein, His, Avitag™ (MALS verified)

Lymphotoxin-beta receptor (LTBR) is also known as Tumor necrosis factor receptor superfamily member 3 (TNFRSF3), Tumor necrosis factor receptor type III (TNF-RIII), which is a single-pass type I membrane protein containing four TNFR-Cys repeat regions. Except for interacting with HCV core protein, LTBR can not only associate with itself, but also can associate with TRAF3, TRAF4 and TRAF5. As the receptor for the heterotrimeric lymphotoxin containing LTA and LTB, and for TNFS14/LIGHT, LTBR promotes apoptosis via TRAF3 and TRAF5. Furthermore, LTBR may play a role in the development of lymphoid organs.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 25.3 kDa. The protein migrates as 34 kDa and 35-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P36941-1

Expression Region

Gln 31 - Met 227

Host

HEK293

Target

LTBR

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Biotinylated Human LTBR, His,Avitag (Cat. No. LTR-H82E9) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human LIGHT, Fc Tag (active trimer) (MALS verified) (Cat. No. LIT-H5269) with a linear range of 0.1-8 ng/mL (QC tested).