Human / Cynomolgus / Rhesus macaque CD28 Protein, Mouse IgG2a Fc Tag (MALS verified)

T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) . When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs) . The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 42.0 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag (Cat. No. CD8-H52A5) at 5 μg/mL (100 μL/well) can bind Human B7-2, Fc Tag (Cat. No. CD6-H5257) with a linear range of 0.1-5 μg/mL (QC tested).