Cynomolgus / Rhesus macaque CD27 / TNFRSF7 Protein, Mouse IgG2a Fc Tag, low endotoxin (MALS verified)

Cynomolgus / Rhesus macaque CD27 Protein, Mouse IgG2a Fc Tag (CD7-C5259) is expressed from human 293 cells (HEK293) . It contains AA Ala 20 - Arg 191 (Accession # XP_005569963.1) . In the region Ala 20 - Arg 191, the AA sequence of Cynomolgus and Rhesus macaque CD27 are homologus.

Product Specifications

Background

CD Antigen CD27 is also known as Tumor necrosis factor receptor superfamily member 7 (TNFRSF7), which belongs to TNF-receptor superfamily. CD27 / TNFRSF7 is found in most T-lymphocytes. CD27 / TNFRSF7 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. CD27 has been shown to interact with SIVA1, TRAF2 and TRAF3.

Specifications

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 46.2 kDa. The protein migrates as 50-66 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

XP_005569963.1

Expression Region

Ala 20 - Arg 191

Host

HEK293

Target

CD27

Conjugation

Unconjugated

Tag

C-mFc (mIgG2a)

Source

Cynomolgus / Rhesus macaque

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.01 EU per μg

Purity

95%

Bioactivity

Immobilized Cynomolgus / Rhesus macaque CD27 Protein, Mouse IgG2a Fc Tag (Cat. No. CD7-C5259) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD27 Ligand, Fc Tag with a linear range of 2-31 ng/mL (QC tested).