Biotinylated Human B7-H4 Protein, Avitag™, His Tag (recommended for biopanning) (MALS verified)

V-set domain-containing T-cell activation inhibitor 1 (VTCN1) is also known as Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7H4, which belongs to the immunoglobulin superfamily and BTN/MOG family. VTCN1 contains two Ig-like V-type (immunoglobulin-like) domains. The expression of VTCN1 is up-regulated by IL6 and IL10 and is inhibited by GM-CSF and IL4 on antigen-presenting cells (APCs) . VTCN1 / B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. VTCN1 involved in promoting epithelial cell transformation.

Product Specifications

Background

Specifications

This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 28 kDa. The protein migrates as 43-57 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

NP_078902

Expression Region

Phe 29 - Ala 258

Host

HEK293

Target

B7-H4

Conjugation

Biotin-labeled

Tag

C-Avi & C-6xHis

Source

Human

Applications

B74-H82E2 works best for experiments that test the binding between B7-H4 and candidate antibodies, such as biopanning and other relevant assays.

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Anti-Human B7-H4 MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human B7-H4 Protein, Avitag,His Tag (Cat. No. B74-H82E2) with a linear range of 3-48 ng/mL (QC tested).