Biotinylated Human B7-H4 Protein, His Tag, ultra sensitivity (primary amine labeling) (MALS verified)

V-set domain-containing T-cell activation inhibitor 1 (VTCN1) is also known as Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7H4, which belongs to the immunoglobulin superfamily and BTN/MOG family. VTCN1 contains two Ig-like V-type (immunoglobulin-like) domains. The expression of VTCN1 is up-regulated by IL6 and IL10 and is inhibited by GM-CSF and IL4 on antigen-presenting cells (APCs) . VTCN1 / B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. VTCN1 involved in promoting epithelial cell transformation.

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.4 kDa. The protein migrates as 43-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Immobilized Anti-Human B7-H4 mAb at 2 μg/mL (100 μL/well) can bind Biotinylated Human B7-H4, His Tag, primary amine labeling (Cat. No. B74-H8222) with a linear range of 1-8 ng/mL (QC tested).