Biotinylated Mouse Fc gamma RIV / CD16-2 Protein, His, Avitag™ (HPLC & BLI verified)

FcgR4 (Low affinity immunoglobulin gamma Fc region receptor IV) is also known as CD16-2, FcgammaRIV, receptor for the Fc region of immunoglobulin gamma . Also acts as a receptor for the Fc region of immunoglobulin epsilon . Binds with intermediate affinity to both IgG2a and IgG2b . Does not display binding to IgG1 or IgG3. Plays a role in promoting bone resorption by enhancing osteoclast differentiation following binding to IgG2a. Binds with low affinity to both the a and b allotypes of IgE. Has also been shown to bind to IgE allotype a only but not to allotype b. Binding to IgE promotes macrophage-mediated phagocytosis, antigen presentation to T cells, production of proinflammatory cytokines and the late phase of cutaneous allergic reactions

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 24.6 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

A0A0B4J1G0-1

Expression Region

Gly 21 - Gln 203

Host

HEK293

Target

Fc gamma RIV / CD16-2

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Mouse

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 12 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized FMC63 at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse CD16-2 Protein, His,Avitag (Cat. No. FC4-M82E8) with a linear range of 0.3-20 ng/mL (Routinely tested).