SARS-CoV GD01 Spike Trimer Protein (R667A, KV968-969PP), His Tag (MALS verified)

SARS-CoV GD01 Spike Trimer, His Tag (SPN-S52Ht) is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. It contains AA Ser 14 - Pro 1195 (Accession # AAP51227.1 (R667A, KV968-969PP) ) .

Product Specifications

Background

The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 136.5 kDa. The protein migrates as 160-190 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAP51227.1 (R667A, KV968-969PP)

Expression Region

Ser 14 - Pro 1195

Host

HEK293

Target

Spike protein

Conjugation

Unconjugated

Tag

C-10xHis

Source

SARS-CoV GD01

Stability

● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind SARS-CoV GD01 Spike Trimer, His Tag (Cat. No. SPN-S52Ht) with a linear range of 0.5-16 ng/mL (QC tested).

Format

Powder

Buffer

0.1 M Sodium citrate, pH5.5

Additives

Trehalose

Molecular Weight

136.5 kDa

Shipping Conditions

Storage Conditions

-20°C

Package Size

50ug*1

Product Datasheet

Https://www.acrobiosystems.com/static/main/products/pdf/ds/SPN-S52Ht.pdf

Species