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Pangolin coronavirus hCoV-19/pangolin/Guangdong/1/2019 Spike Trimer Protein (R677A, KV978-979AA), His Tag

Selected Variant

SPN-P52H3-50ug

Size: 50 µg

Pangolin coronavirus hCoV-19/pangolin/Guangdong/1/2019 Spike Trimer, His Tag (SPN-P52H3) is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. It contains AA Ala 10 - Pro 1205 (Accession # A0A7D6TQ96-1 (R677A, KV978-979AA) ) .

Product Specifications

Background

The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 137.6 kDa. The protein migrates as 170-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

A0A7D6TQ96-1 (R677A, KV978-979AA)

Expression Region

Ala 10 - Pro 1205

Host

HEK293

Target

Spike protein

Conjugation

Unconjugated

Tag

C-10xHis

Source

Pangolin coronavirus

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Pangolin coronavirus hCoV-19/pangolin/Guangdong/1/2019 Spike Trimer, His Tag (Cat. No. SPN-P52H3) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-8 ng/mL (Routinely tested).

Format

Powder

Buffer

0.1 M Sodium citrate, pH5.5

Additives

Trehalose

Molecular Weight

137.6 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

50ug*1

Host or Source

HEK293

Species

Pangolin coronavirus

Protein ID

A0A7D6TQ96-1 (R677A, KV978-979AA)

Preservative

Trehalose

Price

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