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Bat coronavirus hCoV-19/bat/Yunnan/RmYN02/2019 Spike Trimer Protein (R640A, KV940-941PP), His Tag

Selected Variant

SPN-B52H3-50ug

Size: 50 µg

Bat coronavirus hCoV-19/bat/Yunnan/RmYN02/2019 Spike Trimer, His Tag (SPN-B52H3) is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. It contains AA Ala 11 - Pro 1167 (Accession # EPI_ISL_412977 (R640A, KV940-941PP) ) .

Product Specifications

Background

The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 132.5 kDa. The protein migrates as 150-180 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

EPI_ISL_412977 (R640A, KV940-941PP)

Expression Region

Ala 11 - Pro 1167

Host

HEK293

Target

Spike protein

Conjugation

Unconjugated

Tag

C-10xHis

Source

Bat coronavirus

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Format

Powder

Buffer

0.1 M Sodium citrate, pH5.5

Additives

Trehalose

Molecular Weight

132.5 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

50ug*1

Host or Source

HEK293

Species

Bat coronavirus

Protein ID

EPI_ISL_412977 (R640A, KV940-941PP)

Preservative

Trehalose

Price

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