SARS-CoV-2 Nucleocapsid protein, His Tag (BA.4/Omicron)

SARS-CoV-2 Nucleocapsid protein, His Tag (BA.4/Omicron) (NUN-C52Hw) is expressed from human 293 cells (HEK293) . It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (P13L, ERS31-33del, P151S, R203K, G204R, S413R) ) . The mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.4; GISAID clade: GRA) .

Product Specifications

Background

Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68) . The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 47.1 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

QHO62115.1 (P13L, ERS31-33del, P151S, R203K, G204R, S413R)

Expression Region

Met 1 - Ala 419

Host

HEK293

Target

Nucleocapsid protein

Conjugation

Unconjugated

Tag

C-6xHis

Source

SARS-CoV-2

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (BA.4/Omicron) (Cat. No. NUN-C52Hw) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 (Cat. No. NUN-CH15) with a linear range of 0.1-2 ng/mL (QC tested).