Biotinylated SARS Spike RBD Protein, His, Avitag™ (MALS verified)

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 28.6 kDa. The protein migrates as 36-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAP13567.1

Expression Region

Arg 306 - Phe 527

Host

HEK293

Target

Spike RBD

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

SARS

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Biotinylated SARS Spike RBD, His,Avitag (Cat. No. SPD-S82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-8 ng/mL (QC tested).