Biotinylated SARS-CoV-2 (COVID-19) Nucleocapsid protein, His, Avitag™

Biotinylated SARS-CoV-2 Nucleocapsid protein, His, Avitag (NUN-C81Q6) is expressed from E. coli cells. It contains AA Met 1 - Ala 419 (Accession # QHO62115.1) .

Product Specifications

Background

Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68) . The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

Specifications

This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 51.1 kDa. The protein migrates as 52 kDa under reducing (R) condition (SDS-PAGE) .

Accession Number

QHO62115.1

Expression Region

Met 1 - Ala 419

Host

E. coli

Target

Nucleocapsid protein

Conjugation

Biotin-labeled

Tag

N-6xHis & N-Avi

Source

SARS-CoV-2

Stability

● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Biotinylated SARS-CoV-2 Nucleocapsid protein, His,Avitag (Cat. No. NUN-C81Q6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 (Cat. No. NUN-CH15) with a linear range of 0.1-2 ng/mL (QC tested).