Biotinylated Mouse CD3 epsilon&CD3 gamma Heterodimer Protein, His, Avitag™&Flag Tag (MALS verified)

T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Product Specifications

Background

Specifications

Biotinylated Mouse CD3E&CD3G Heterodimer Protein, His, Avitag&Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 16.8 kDa (CD3E) and 15.2 kDa (CD3G) . Subunit CD3E carries a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit CD3G is fused with a flag tag at the C-terminus. As a result of glycosylation, the protein migrates as 20-23 kDa under reducing (R) condition, and 35-40 kDa under non-reducing (NR) condition (SDS-PAGE) .

Accession Number

P22646-1 (CD3E) & P11942-1 (CD3G)

Expression Region

Asp 23 - Asp 108 (CD3E) & Gln 23 - Ser 116 (CD3G)

Host

HEK293

Target

CD3E & CD3G

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi | C-Flag

Source

Mouse

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized CD3E Monoclonal Antibody (145-2C11) at 1 μg/mL (100 μL/well) can bind Biotinylated Mouse CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag (Cat. No. CDG-M58D2) with a linear range of 5-78 ng/mL (QC tested).