DENV2 (strain Thailand/16681/1984) NS1 protein, His Tag (MALS verified)

Dengue is a mosquito-borne viral disease widely spread all over the world transmitted by 4 serotypes of dengue virus (DENV) . The symptoms range from mild dengue fever to dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) with high mortality. Currently, most of the studies on DENV vaccines emphasize on its envelope protein (i.e., Sanofi’s tetravalent dengue vaccine Dengvaxia) . However, the fact pointing out that the vaccines with Envelope protein may could have negative effect on people never infected by DENV which makes the non-structural protein NS1 become another great target for vaccine research with its ability to avoid Antibody Dependent Enhancement.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 41.8 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P29990-1

Expression Region

Asp 776 - Ala 1127

Host

HEK293

Target

Non-structural protein 1/NS1 (DENV)

Conjugation

Unconjugated

Tag

C-10xHis

Source

Dengue virus

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized DENV2 (strain Thailand/16681/1984) NS1 protein, His Tag (Cat. No. NS1-D52H3) at 1 μg/mL (100 μL/well) can bind Mouse Anti-NS1(DENV) Antibody 2B7,Mouse IgG1 with a linear range of 0.1-2 ng/mL (QC tested).