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Human CD3 epsilon&CD3 gamma Heterodimer Protein, Fc, His Tag&Fc, Flag Tag (MALS verified)

Selected Variant

CDG-H52W9-50ug

Size: 50 µg

T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Product Specifications

Background

T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Specifications

Human CD3E&CD3G Heterodimer Protein, Fc, His Tag&Fc, Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3G) . Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag, and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus, followed by a Flag tag. The predicted N-terminus is Asp 23 (CD3E) & Gln 23 (CD3G) . The reducing (R) heterodimer protein migrates as 40-50 kDa due to glycosylation.

Accession Number

P07766-1 (CD3E) & P09693-1 (CD3G)

Expression Region

Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G)

Host

HEK293

Target

CD3E & CD3G

Conjugation

Unconjugated

Tag

C-Fc & C-6xHis | C-Fc & C-Flag

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-H52W9) at 2 μg/mL (100 μL/well) can bind UCHT1 with a linear range of 0.4-6 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

39.2 kDa (CD3E) & 38.6 kDa (CD3G)

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

50ug*1

Host or Source

HEK293

Species

Human

Protein ID

P07766-1 (CD3E) & P09693-1 (CD3G)

Preservative

Trehalose

Price

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