Human CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin

T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Product Specifications

Background

Specifications

Human CD3E & CD3D Heterodimer Protein, Llama IgG2b Fc Tag & Llama IgG2b Fc Tag, low endotoxin is produced by co-expression of CD3E and CD3D, has a calculated MW of 42.9 kDa (CD3E) and 40.8 kDa (CD3D) . Subunit CD3E is fused with a llama IgG2b Fc tag at the C-terminus and subunit CD3D is fused with a llama IgG2b Fc tag at the C-terminus. The predicted N-terminus is Asp 23 (CD3E) & Phe 22 (CD3D) . The reducing (R) protein migrates as 50-60 kDa due to glycosylation.

Accession Number

P07766-1 (CD3E) & P04234-1 (CD3D)

Expression Region

Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D)

Host

HEK293

Target

CD3E & CD3D

Conjugation

Unconjugated

Tag

C-LlamaFc (LlamaIgG2b) | C-LlamaFc (LlamaIgG2b)

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.01 EU per μg

Purity

95%

Bioactivity

Immobilized Human CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc (Cat. No. CDD-H5258) at 2 μg/mL (100 μL/well) can bind SP34-2 (mouse IgG1) with a linear range of 1-20 ng/mL (Routinely tested).

Format

Powder

Buffer

Tris with Glycine, Arginine and NaCl, pH7.5

Additives

Trehalose

Molecular Weight

42.9 kDa (CD3E) & 40.8 kDa (CD3D)

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

Storage Conditions

-20°C

Package Size

100ug*1

Host or Source

HEK293

Species

Human

Protein ID

P07766-1 (CD3E) & P04234-1 (CD3D)

Preservative

Trehalose

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

32019-05141 2x 75 µg

Human Spermidine Synthase (SPDSY) AssayLite™ Antibody (FITC Conjugate)

Ask

View Details

bs-7694R-Biotin 100 µL

SLC12A3 Polyclonal Antibody, Biotin Conjugated

Ask

View Details

TRC-P359480-500MG 500 mg

Phosphoric Acid Diethyl Octyl Ester

Ask

View Details

E47R12824 100 µL

STEAP2 Rabbit pAb

Ask

View Details

GTR15255184 1 Vial

Lentiviral mouse Sar1b shRNA (UAS) - Lentiviral mouse Sar1b shRNA (UAS, iRFP) plasmid

Ask

View Details

429378-01 100 mg

4- (Boc-amino) -1-cyclopentylpiperidine

Ask

View Details