Cynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin

T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Product Specifications

Background

Specifications

Cynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc is produced by co-expression of CD3E and CD3D, has a calculated MW of 41.9 kDa (CD3E) and 40.8 kDa (CD3D) . Subunit CD3E is fused with a llama IgG2b Fc tag at the C-terminus and subunit CD3D is fused with a llama IgG2b Fc tag at the C-terminus. The predicted N-terminus is Gln 22 (CD3E) & Phe 22 (CD3D) . The reducing (R) protein migrates as 50 kDa and 55-65 kDa due to glycosylation.

Accession Number

Q95LI5-1 (CD3E) & Q95LI8-1 (CD3D)

Expression Region

Gln 22 - Asp 117 (CD3E) & Phe 22 - Ala 105 (CD3D)

Host

HEK293

Target

CD3E & CD3D

Conjugation

Unconjugated

Tag

C-LlamaFc (LlamaIgG2b) | C-LlamaFc (LlamaIgG2b)

Source

Cynomolgus

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.01 EU per μg

Purity

90%

Bioactivity

Cynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc (Cat. No. CDD-C5259) at 1 μg/mL (100 μL/well) can bind Mouse Anti-Human CD3 (SP34-2) (mouse IgG1) with a linear range of 2-63 ng/mL (QC tested).