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Human CD3 epsilon&CD3 delta Heterodimer Protein, Fc, His Tag&Fc, Flag Tag (MALS verified)

T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Product Specifications

Background

T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Specifications

Human CD3E&CD3D Heterodimer Protein is produced by co-expression of CD3E and CD3D, has a calculated MW of 40.2 kDa (CD3E) and 37.3 kDa (CD3D) . Subunit CD3E is fused with a human IgG1 Fc tag and a polyhistidine tag at the C-terminus and subunit CD3D is fused with a human IgG1 Fc tag and a flag tag at the C-terminus. As a result of glycosylation, the heterodimer protein migrates as 45-60 kDa under reducing (R) condition, and 100-116 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) .

Accession Number

P07766-1 (CD3E) & P04234-1 (CD3D)

Expression Region

Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D)

Host

HEK293

Target

CD3E & CD3D

Conjugation

Unconjugated

Tag

C-Fc & C-10xHis | C-Fc & C-Flag

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. After reconstitution, this product is stable after storage at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human CD3E&CD3D Heterodimer Protein (Cat. No. CDD-H52Wa) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a) with a linear range of 0.2-3 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

40.2 kDa & 37.3 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

100ug*1

Host or Source

HEK293

Species

Human

Protein ID

P07766-1 (CD3E) & P04234-1 (CD3D)

Preservative

Trehalose

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