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Cynomolgus CD3 epsilon&CD3 delta Heterodimer Protein, Fc, His Tag&Fc, Flag Tag (MALS verified)

Selected Variant

CDD-C52W9-100ug

Size: 100 µg

T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Product Specifications

Background

T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Specifications

Cynomolgus CD3E&CD3D Heterodimer Protein, Fc, His Tag&Fc, Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 42.5 kDa (CD3E) and 40.9 kDa (CD3D) . Subunit CD3E is fused with a human IgG1 Fc tag and a polyhistidine tag at the C-terminus and subunit CD3D is fused with a human IgG1 Fc tag and a flag tag at the C-terminus. The predicted N-terminus is Gln 22 (CD3E) & Phe 22 (CD3D) . The reducing (R) protein migrates as 46-50 kDa and 55-65 kDa due to glycosylation.

Accession Number

Q95LI5-1 (CD3E) & Q95LI8-1 (CD3D)

Expression Region

Gln 22 - Asp 117 (CD3E) & Phe 22 - Ala 105 (CD3D)

Host

HEK293

Target

CD3E & CD3D

Conjugation

Unconjugated

Tag

C-Fc & C-10xHis | C-Fc & C-Flag

Source

Cynomolgus

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Bispecific CD3×BCMA T cell-engaging Antibody at 1 μg/mL (100 μL/well) can bind Cynomolgus CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-C52W9) with a linear range of 1-20 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

42.5 kDa (CD3E) & 40.9 kDa (CD3D)

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

100ug*1

Host or Source

HEK293

Species

Cynomolgus

Protein ID

Q95LI5-1 (CD3E) & Q95LI8-1 (CD3D)

Preservative

Trehalose

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