Biotinylated Mouse IL-6 Protein, Avitag™, His Tag

Interleukin 6 (IL-6) is also known as HGF, BSF2, HSF, IFNB2 and IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine that regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.It is secreted by T cells, macrophages , monocytes, fibroblasts, endothelial cells, et.al. to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein. IL-6 is relevant to many disease processes such as diabetes, atherosclerosis, depression, Alzheimer's Disease, systemic, lupus erythematosus, prostate cancer and rheumatoid arthritis. Advanced/metastatic cancer patients have higher levels of IL-6 in their blood.Hence there is an interest in developing anti-IL-6 agents as therapy against many of these diseases.

Product Specifications

Background

Specifications

This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 25.3 kDa. The protein migrates as 28-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P08505-1

Expression Region

Phe 25 - Thr 211

Host

HEK293

Target

IL-6

Conjugation

Biotin-labeled

Tag

N-Avi & N-10xHis

Source

Mouse

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Biotinylated Mouse IL-6, Avitag,His Tag (Cat. No. IL6-M82Q7) at 1 μg/mL (100 μL/well) on streptavidin precoated (STN-N5116) can bind Mouse IL-6 R alpha Protein, Fc Tag (Cat. No. ILR-M5255) with a linear range of 0.3-2 ng/mL (QC tested).