Biotinylated Human Integrin isoform alpha-7X2B beta 1 (ITGA7X2B&ITGB1) Heterodimer Protein, Avitag™&His, Avitag™

Integrin alpha-7/beta-1 is the primary laminin receptor on skeletal myoblasts and adult myofibers, which is highly expressed in cardiac muscle, skeletal muscle and smooth muscle cells, and localizes to Z-disc and costamere structures. It is involved in the maintenance of the myofibers cytoarchitecture as well as for their anchorage, viability and functional integrity. Isoform Alpha-7X2B and isoform Alpha-7X1B promote myoblast migration on laminin 1 and laminin 2/4, but isoform Alpha-7X1B is less active on laminin 1 (In vitro) . ITGA7 has been shown to interact with Merosin, ITGB1, FHL2 and FHL3.

Product Specifications

Background

Specifications

Biotinylated Human ITGA7X2B & ITGB1 Heterodimer Protein, produced by co-expression of ITGA7X2B and ITGB1, has a calculated MW of 116.9 kDa (ITGA7X2B) and 86.7 kDa (ITGB1) . Subunit ITGA7X2B is fused with an acidic tail and an Avi tag (Avitag™) at the C-terminus and subunit ITGB1 contains a basic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) . The non-reducing (NR) protein migrates as 100-115 kDa due to glycosylation.

Accession Number

Q13683-7 (ITGA7X2B) & P05556-1 (ITGB1)

Expression Region

Phe 34 - Trp 1038 (ITGA7X2B) & Gln 21 - Asp 728 (ITGB1)

Host

HEK293

Target

Integrin isoform alpha-7X2B beta 1

Conjugation

Biotin-labeled

Tag

Native | C-10xHis & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.