Human IL-23 alpha&Rat IL-12 beta Heterodimer Protein, His Tag&Tag Free (MALS verified)

Interleukin-23 subunit alpha (IL-23 alpha) can associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

Product Specifications

Background

Specifications

Human IL-23A & Rat IL-12B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of IL-23A and IL-12B, has a calculated MW of 20.6 kDa (IL-23A) and 35.9 kDa (IL-12B) . Subunit IL-23A is fused with a polyhistidine tag at the N-terminus and subunit IL-12B contains no tag. The reducing (R) protein migrates as 23 kDa (IL-23A) and 45 kDa and 47 kDa (IL-12B) respectively due to glycosylation.

Accession Number

Q9NPF7-1 (IL23A) & Q9R278-1 (IL12B)

Expression Region

Arg 20 - Pro 189 (IL23A) & Met 23 - Ser 335 (IL12B)

Host

HEK293

Target

IL23A & IL12B

Conjugation

Unconjugated

Tag

N-10xHis | Native

Source

Human & Rat

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human IL-23 R, Fc Tag (Cat. No. ILR-H5254) at 5 μg/mL (100 μL/well) can bind Human IL-23A & Rat IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HR52W3) with a linear range of 0.039-0.156 μg/mL (QC tested).