Biotinylated Mouse VEGF120 Protein, His, Avitag™

Vascular endothelial growth factor A (VEGFA) is also known as Vascular permeability factor (VPF) . VEGFA belongs to the PDGF/VEGF growth factor family. VEGFA is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. VEGFA is produced by a group of three major isoforms as a result of alternative splicing and if any three isoforms are produced (VEGFA120, VEGFA164, and VEGFA188) then this will not result in vessel defects and death of the full VEGFA knockout in mice.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 17.3 kDa. The protein migrates as 22-24 kDa and 24-26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q00731-3

Expression Region

Ala 27 - Arg 146

Host

HEK293

Target

VEGF120

Conjugation

Biotin-labeled

Tag

N-10xHis & N-Avi

Source

Mouse

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized VEGFR1/R2 at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse VEGF120, His,Avitag (Cat. No. VE0-M82Q2) with a linear range of 0.1-2 ng/mL (QC tested).