Human Siglec-9 Protein, His Tag
Human Siglec-9, His Tag (SI9-H52H4) is expressed from human 293 cells (HEK293) . It contains AA Gln 18 - Gly 348 (Accession # Q9Y336-1) .
Product Specifications
Background
Siglec-9 (HGMW-approved symbol SIGLEC9) a member of the sialic acid-binding Ig-like lectin (Siglec) family, which belongs to the immunoglobulin superfamily (IgSF) . SIGLEC9 shows a high degree of homology to many members of the siglec family, including siglec-7 (80%), siglec-8 (72%), siglec-5 (65%), and CD33 (64%) . This high degree of homology is also conserved in the extracellular Ig-like domains. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains。Siglec-9 with a hydrophobic signal peptide, an N-terminal Ig-likeV-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 37.9 kDa. The protein migrates as 53-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
Q9Y336-1
Expression Region
Gln 18 - Gly 348
Host
HEK293
Target
Siglec-9
Conjugation
Unconjugated
Tag
C-10xHis
Source
Human
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
90%
Bioactivity
Immobilized Human Siglec-9, His Tag (Cat. No. SI9-H52H4) at 10 μg/mL (100 μL/well) on Nickel Coated plate, can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with a linear range of 0.078-2.5 μg/mL (QC tested).
Format
Powder
Buffer
25 mM MES, 150 mM NaCl, pH5.5
Additives
Trehalose
Molecular Weight
37.9 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
100ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/SI9-H52H4.pdf
Species
Human
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