Cynomolgus / Rhesus macaque TIGIT Protein, Mouse IgG2a Fc Tag (MALS verified)

T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3), which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM) . TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level) . TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

Product Specifications

Background

Specifications

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 40.2 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

G7NXM4-1

Expression Region

Met 89 - Ile 208

Host

HEK293

Target

TIGIT

Conjugation

Unconjugated

Tag

C-mFc (mIgG2a)

Source

Cynomolgus / Rhesus macaque

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.1 EU per μg

Purity

90%

Bioactivity

Immobilized Rhesus macaque CD155, His Tag (Cat. No. CD5-R52H5) at 5 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque TIGIT, Mouse IgG2a Fc Tag (Cat. No. TIT-C5253) with a linear range of 0.3-20 ng/mL (QC tested).