Mouse TIGIT Protein, Mouse IgG2a Fc Tag (MALS verified)

Mouse TIGIT, Mouse IgG2a Fc Tag (TIT-M5253) is expressed from human 293 cells (HEK293). It contains AA Gly 26 - Thr 143 (Accession # NP_001139797.1).

Product Specifications

Background

T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

Specifications

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 39.8 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Gly 26 - Thr 143

Host

HEK 293

Target

TIGIT

Conjugation

Unconjugated

Tag

C-mFc(mIgG2a)

Source

Mouse

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.1 EU per μg

Purity

95%

Bioactivity

Immobilized Biotinylated Mouse CD155, Fc,Avitag (Cat. No. CD5-M82F7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse TIGIT, Mouse IgG2a Fc Tag (Cat. No. TIT-M5253) with a linear range of 0.1-2 ng/mL (QC tested).