Human CD58 / LFA-3 Protein, His Tag

CD antigen CD58 is also known as Lymphocyte function-associated antigen 3 (LFA-3) . CD58 is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. CD58 /LFA3 is ligand of the T-lymphocyte CD2 glycoprotein, which binds to CD2 (LFA-2) [3] on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells. Polymorphisms in the CD58 gene are associated with increased risk for multiple sclerosis.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 22.3 kDa. The protein migrates as 30-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAH05930

Expression Region

Phe 29 - Arg 215

Host

HEK293

Target

CD58

Conjugation

Unconjugated

Tag

C-6xHis

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human CD58, His Tag (Cat. No. LF3-H5225) at 10 μg/mL (100 μL/well) can bind Human CD2, Fc Tag (Cat. No. CD2-H5258) with a linear range of 5-78 ng/mL (QC tested).