Human Integrin alpha 4 beta 7 (ITGA4&ITGB7) Heterodimer Protein, His Tag&Tag Free, low endotoxin (MALS & SPR verified)

Integrin alpha 4 beta 7 is an integrin family adhesion receptor that shares subunits with alpha 4 beta 1 (VLA4) and the E-Cadherin receptor, alpha E beta 7 . Integrin alpha 4 beta 7, which is critical for homing to intestinal mucosa, is induced during T cell activation in Peyer’s patches or mesenteric lymph nodes.Its expression requires signals from local dendritic and stromal cells, including secreted retinoic acid. The HIV-1 envelope protein gp120 binds to the active form of Integrin alpha 4 beta 7, and this may or may not account for the concentration of HIV-1 virus in the gut-associated lymphoid tissue (GALT) . Integrin alpha 4 beta 7 may also be involved in lymphocyte trafficking in acute intestinal graft vs. host disease (GVHD) .

Product Specifications

Background

Specifications

Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA4 and ITGB7, has a calculated MW of 111.4 kDa (ITGA4) & 83.7 kDa (ITGB7) . Subunit ITGA4 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB7 contains no tag but a basic tail at the C-terminus. The protein migrates as 130-150 kDa (ITGA4) and 65 kDa, 70 kDa, 100-120 kDa (ITGB7) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Accession Number

P13612-1 (ITGA4) & P26010-1 (ITGB7)

Expression Region

Tyr 34 - Thr 977 (ITGA4) & Glu 20 - His 723 (ITGB7)

Host

HEK293

Target

Integrin alpha 4 beta 7

Conjugation

Unconjugated

Tag

C-10xHis | Native

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.01 EU per μg

Purity

90%

Bioactivity

Immobilized Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT7-H52W4) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human ITGA4&ITGB7 Antibody, Human IgG4 with a linear range of 0.1-2 ng/mL (Routinely tested).