Biotinylated Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein, His, Avitag™&Tag Free (MALS verified)

Integrin alpha V beta 5 (ITGAV & ITGB5) is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, embryonic stem cells, and select endothelium and epithelium. ITGAV & ITGB5 binds ligands containing an RGD motif, notably vitronectin. Growth factors that increase PKC activity, such as VEGF or TGF alpha, promote ITGAV & ITGB5-mediated angiogenesis while alpha V beta 3, which may be expressed in the same cell, responds to FGF-basic and TNF alpha. An inhibitor of both down regulates tumor angiogenesis. During lung inflammation, up regulation of ITGAV & ITGB5 on myofibroblasts or infiltrating lymphocytes may contribute to fibrosis by freeing TGF beta from latency.

Product Specifications

Background

Specifications

Biotinylated Human ITGAV&ITGB5 Heterodimer Protein, His, Avitag&Tag Free, produced by co-expression of ITGAV and ITGB5, has a calculated MW of 114.7 kDa (ITGAV) and 81.9 kDa (ITGB5) . Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB5 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 130-170 kDa (ITGAV) and 85-100 kDa (ITGB5) when calibrated against Star Ribbon Pre-stained Protein Marker respectively due to glycosylation.

Accession Number

P06756-1 (ITGAV) & P18084-1 (ITGB5)

Expression Region

Phe 31 - Val 992 (ITGAV) & Gly 24 - Asn 719 (ITGB5)

Host

HEK293

Target

Integrin alpha V beta 5

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi | Native

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human Vitronectin Protein, Tag Free at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB5 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT5-H82Wa) with a linear range of 1-31 ng/mL (Routinely tested).