Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein, Tag Free&Tag Free

Integrin alpha-5/beta-1 is a receptor for ibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-V/beta-1 is also a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro) . When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition.

Product Specifications

Background

Specifications

Human ITGAV&ITGB1 Heterodimer Protein, Tag Free&Tag Free, produced by co-expression of ITGAV and ITGB1, has a calculated MW of 112.3 kDa (ITGAV) and 83.7 kDa (ITGB1) . Subunit ITGAV contains no tag but an acidic tail at the C-terminus and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The protein migrates as 130-150 kDa (ITGAV) and 100-115 kDa (ITGB1) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (NR) condition (SDS-PAGE) due to glycosylation.

Accession Number

NP_002201.1 (ITGAV) & NP_002202.2 (ITGB1)

Expression Region

Phe 31 - Val 992 (ITGAV) & Gln 21 - Asp 728 (ITGB1)

Host

HEK293

Target

Integrin alpha V beta 1

Conjugation

Unconjugated

Tag

Native | Native

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Recombinant Human Fibronectin at 2 μg/mL (100 μL/well) can bind Human ITGAV&ITGB1 Heterodimer Protein, Tag Free&Tag Free (Cat. No. IT1-H5213) with a linear range of 0.002-0.313 μg/mL (QC tested).