Human IL-6 R alpha / CD126 Protein, Fc Tag

Interleukin 6 receptor (IL6R) is also known as CD126 (Cluster of Differentiation 126), is a potent pleiotropic cytokine that regulates cell growth and differentiation of various tissues, and is known particularly for its role in the immune response and acute phase reactions. IL6R is a protein complex consisting of a IL-6 receptor subunit (IL6R) and interleukin 6 signal transducer Glycoprotein 130. IL6R also denotes the human gene encoding this subunit. Alternatively spliced transcript variants encoding distinct isoforms have been reported. IL6R subunit also shared by many other cytokines. The soluble form of IL6R arises from proteolytic cleavage of membrane-bound IL6Rα, and acts agonistically by making the IL6 ligand accessible to the signal transducer gp130. Dysregulated production of IL6 and IL6R are implicated in the pathogenesis of several inflammatory diseases and malignancies such as multiple myeloma, rheumatoid arthritis, or osteoporosis, and it has been reported that a humanized anti-IL6R monoclonal antibody is a promising agent applicable to the therapeutic approach for IL6 driven diseases. Interleukin-6 receptor has been shown to interact with Interleukin 6 and Ciliary neurotrophic factor.

Product Specifications

Background

Specifications

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 65.0 kDa. The protein migrates as 70-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P08887-1

Expression Region

Leu 20 - Pro 365

Host

HEK293

Target

IL-6 R alpha

Conjugation

Unconjugated

Tag

C-Fc

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human IL-6, premium grade (Cat. No. IL6-H4218) at 5 μg/mL (100 μL/well) can bind Human IL-6 R alpha, Fc Tag (Cat. No. ILR-H5259) with a linear range of 1-20 ng/mL (QC tested).