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Biotinylated Human IL-2 R beta&IL-2 R gamma Heterodimer Protein, Fc, Avitag™&Fc, Avitag™ (MALS verified)

Selected Variant

ILG-H82F3-25ug

Size: 25 µg

Both Interleukin-2 receptor subunit beta and Interleukin-2 receptor subunit gamma are receptor for interleukin-2. Common subunit for the receptors for a variety of interleukins. Interacts with SHB upon interleukin stimulation. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit) . The high and intermediate affinity forms also associate with a gamma subunit.

Product Specifications

Background

Both Interleukin-2 receptor subunit beta and Interleukin-2 receptor subunit gamma are receptor for interleukin-2. Common subunit for the receptors for a variety of interleukins. Interacts with SHB upon interleukin stimulation. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit) . The high and intermediate affinity forms also associate with a gamma subunit.

Specifications

Biotinylated Human IL-2RB&IL-2RG Heterodimer Protein, Fc, Avitag&Fc, Avitag is produced by co-expression of IL-2RB and IL-2RG, has a calculated MW of 52.8 kDa (IL-2RB) and 55.3 kDa (IL-2RG) . Subunit IL-2RB is fused with a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit IL-2RG is fused with a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein migrates as 60-66 kDa and 80-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P14784-1 (IL-2RB) & P31785-1 (IL-2RG)

Expression Region

Ala 27 - Asp 239 (IL-2RB) & Leu 23 - Asn 254 (IL-2RG)

Host

HEK293

Target

IL-2 R beta & IL-2 R gamma

Conjugation

Biotin-labeled

Tag

C-Fc & C-Avi | C-Fc & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human IL-2, Tag Free at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2RB&IL-2RG Heterodimer Protein, Fc,Avitag&Fc,Avitag (Cat. No. ILG-H82F3) with a linear range of 0.01-0.156 μg/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

52.8 kDa & 55.3 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

25ug*1

Host or Source

HEK293

Species

Human

Protein ID

P14784-1 (IL-2RB) & P31785-1 (IL-2RG)

Preservative

Trehalose

Price

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