Cynomolgus IL-23 alpha&Mouse IL-12 beta Heterodimer Protein, His Tag&Tag Free (MALS verified)

Interleukin-23 subunit alpha (IL-23 alpha) can associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

Product Specifications

Background

Specifications

This protein carries polyhistidine tag at the N-terminus. The protein has a calculated MW of 20.3 kDa (IL23A) & 35.8 kDa (IL12B) . The protein migrates as 20-23 kDa (IL23A) & 40-42 kDa and 43-46 kDa (IL12B) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

A0A2K5TLV4-1 (IL23A) & P43432-1 (IL12B)

Expression Region

Val 22 - Pro 189 (IL23A) & Met 23 - Ser 335 (IL12B)

Host

HEK293

Target

IL23A & IL12B

Conjugation

Unconjugated

Tag

N-10xHis | Native

Source

Cynomolgus & Mouse

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Biotinylated Human IL-23 R, Fc,Avitag (Cat. No. ILR-H82F3) at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate can bind Cynomolgus IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-CM52W8) with a linear range of 0.03-0.25 μg/mL (QC tested).